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primary antibodies against type ii collagen, aggrecan, il-1β, and mmp-13  (GeneTex)

 
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    GeneTex primary antibodies against type ii collagen, aggrecan, il-1β, and mmp-13
    Primary Antibodies Against Type Ii Collagen, Aggrecan, Il 1β, And Mmp 13, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+type+ii+collagen+and+aggrecan/anti+mmp13/pm40332404-393-19-21
    Average 90 stars, based on 1 article reviews
    primary antibodies against type ii collagen, aggrecan, il-1β, and mmp-13 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Therapeutic Potential of Olfactory Ensheathing Cells and Adipose-Derived Stem Cells in Osteoarthritis: Insights from Preclinical Studies
    Article Snippet: .. Subsequently, the sections were blocked with Ultra V block (Thermo Scientific) for 10 min and then incubated with primary antibodies against type II collagen and aggrecan (1:200, GeneTex, Irvine, CA, USA) at 37 °C for 4 h. The secondary antibodies used were biotin-labeled goat anti-rabbit immunoglobulin (Dako, Carpinteria, CA, USA) and horseradish peroxidase-conjugated streptavidin (Biocare Medical), which were incubated for 30 min. .. Finally, the sections were stained with 3,3-diaminobenzidine solution, and hematoxylin (Sigma) was used to counterstain the slides.

    Incubation:

    Article Title: Therapeutic Potential of Olfactory Ensheathing Cells and Adipose-Derived Stem Cells in Osteoarthritis: Insights from Preclinical Studies
    Article Snippet: .. Subsequently, the sections were blocked with Ultra V block (Thermo Scientific) for 10 min and then incubated with primary antibodies against type II collagen and aggrecan (1:200, GeneTex, Irvine, CA, USA) at 37 °C for 4 h. The secondary antibodies used were biotin-labeled goat anti-rabbit immunoglobulin (Dako, Carpinteria, CA, USA) and horseradish peroxidase-conjugated streptavidin (Biocare Medical), which were incubated for 30 min. .. Finally, the sections were stained with 3,3-diaminobenzidine solution, and hematoxylin (Sigma) was used to counterstain the slides.



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    Image Search Results


    Adipose stem cell (ASC) characterization. ( A , B ) morphology of ADSC. Scale bar = 100 μm. ( C ) Flowcytometry of ASC. Positive for CD44, CD73, CD90, CD105, and HLA-ABC and negative for CD34, CD45, and HLA-DR. ( D – F ) qRT-PCR showed the gene expressions of adipogenesis ( FABP4 , PPARγ ) ( D ), osteogenesis ( ALPL , RUNX2 ) ( E ), and chondrogenesis (aggrecan and COL2A1 ) ( F ). ( G ) Oil red staining of differentiated cells after adipogenesis. ( H ) Quantification of Oil Red at OD 510. ( I ) Alizarin Red staining of differentiated cells after osteogenesis. ( J ) Quantification of Alizarin Red at OD552. ( K – O ) Characterization of differentiated cell after chondrogenesis. Pellet morphology ( K ), H & E ( L ), Safranin O ( M ), aggrecan ( N ), and type II collagen ( O ). ** p < 0.01, *** p < 0.001. Scale bar = 100 μm.

    Journal: Cells

    Article Title: Therapeutic Potential of Olfactory Ensheathing Cells and Adipose-Derived Stem Cells in Osteoarthritis: Insights from Preclinical Studies

    doi: 10.3390/cells13151250

    Figure Lengend Snippet: Adipose stem cell (ASC) characterization. ( A , B ) morphology of ADSC. Scale bar = 100 μm. ( C ) Flowcytometry of ASC. Positive for CD44, CD73, CD90, CD105, and HLA-ABC and negative for CD34, CD45, and HLA-DR. ( D – F ) qRT-PCR showed the gene expressions of adipogenesis ( FABP4 , PPARγ ) ( D ), osteogenesis ( ALPL , RUNX2 ) ( E ), and chondrogenesis (aggrecan and COL2A1 ) ( F ). ( G ) Oil red staining of differentiated cells after adipogenesis. ( H ) Quantification of Oil Red at OD 510. ( I ) Alizarin Red staining of differentiated cells after osteogenesis. ( J ) Quantification of Alizarin Red at OD552. ( K – O ) Characterization of differentiated cell after chondrogenesis. Pellet morphology ( K ), H & E ( L ), Safranin O ( M ), aggrecan ( N ), and type II collagen ( O ). ** p < 0.01, *** p < 0.001. Scale bar = 100 μm.

    Article Snippet: Subsequently, the sections were blocked with Ultra V block (Thermo Scientific) for 10 min and then incubated with primary antibodies against type II collagen and aggrecan (1:200, GeneTex, Irvine, CA, USA) at 37 °C for 4 h. The secondary antibodies used were biotin-labeled goat anti-rabbit immunoglobulin (Dako, Carpinteria, CA, USA) and horseradish peroxidase-conjugated streptavidin (Biocare Medical), which were incubated for 30 min.

    Techniques: Quantitative RT-PCR, Staining

    Immunohistochemistry of type II collagen and aggrecan of the mouse osteoarthritis model after 28 days of experiments. ( A ) A representative image of type II collagen is illustrated from the normal knee ( n = 4), osteoarthritis (OA) ( n = 4), 5 × 10 5 olfactory ensheathing cells (OECs), 1 × 10 5 OECs, and 1 × 10 5 adipose-derived stem cells (ADSCs) ( n = 4 in each group). ( B ) Quantitative analysis of positive staining cells in 50 cells in five fields. ( C ) A representative image of aggrecan is illustrated from the normal knee ( n = 4), OA ( n = 4), 5 × 10 5 OECs, 1 × 10 5 OECs, and 1 × 10 5 ADSCs ( n = 4 in each group). ( D ) Quantitative analysis of positive staining cells in 50 cells in five fields. * p < 0.05, ** p < 0.01.

    Journal: Cells

    Article Title: Therapeutic Potential of Olfactory Ensheathing Cells and Adipose-Derived Stem Cells in Osteoarthritis: Insights from Preclinical Studies

    doi: 10.3390/cells13151250

    Figure Lengend Snippet: Immunohistochemistry of type II collagen and aggrecan of the mouse osteoarthritis model after 28 days of experiments. ( A ) A representative image of type II collagen is illustrated from the normal knee ( n = 4), osteoarthritis (OA) ( n = 4), 5 × 10 5 olfactory ensheathing cells (OECs), 1 × 10 5 OECs, and 1 × 10 5 adipose-derived stem cells (ADSCs) ( n = 4 in each group). ( B ) Quantitative analysis of positive staining cells in 50 cells in five fields. ( C ) A representative image of aggrecan is illustrated from the normal knee ( n = 4), OA ( n = 4), 5 × 10 5 OECs, 1 × 10 5 OECs, and 1 × 10 5 ADSCs ( n = 4 in each group). ( D ) Quantitative analysis of positive staining cells in 50 cells in five fields. * p < 0.05, ** p < 0.01.

    Article Snippet: Subsequently, the sections were blocked with Ultra V block (Thermo Scientific) for 10 min and then incubated with primary antibodies against type II collagen and aggrecan (1:200, GeneTex, Irvine, CA, USA) at 37 °C for 4 h. The secondary antibodies used were biotin-labeled goat anti-rabbit immunoglobulin (Dako, Carpinteria, CA, USA) and horseradish peroxidase-conjugated streptavidin (Biocare Medical), which were incubated for 30 min.

    Techniques: Immunohistochemistry, Derivative Assay, Staining